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| Registros recuperados: 57 | |
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| Álvarez,Elba; Iglesia,Aleika. |
| Information about the characterization and diagnostic of wild fire was compiled. This bacterial disease, exotic for Cuba, is caused by Pseudomonas syringae pv. tabaci. The species Pseudomonas syringae is traditionally divided into pathovars depending on the host range and symptoms. For its study, different phenotypic and genotypic methods are used. Such methods allow the traditional taxonomic confirmation of this pathovar within the species. Serological and molecular techniques such as: ELISA tests, immunofluorescence, polymerase chain reaction (PCR) are also used for the characterization and detection of the bacterium. Such techniques are very useful and sensible for the diagnostic. |
| Tipo: Journal article |
Palavras-chave: Pseudomonas syringae pv. tabaci; Rep-PCR; Pathogenicity; Serology. |
| Ano: 2007 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000300001 |
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| Andrade,Márcia Cristina Ribeiro; Coelho,Janice Mery Chicarino de Oliveira; Amendoeira,Maria Regina Reis; Vicente,Regiane Trigueiro; Cardoso,Célia Virgínia Pereira; Ferreira,Paola Cristina Brandão; Marchevsky,Renato Sergio. |
| Necropsy findings in three cases of naturally occurring toxoplasmosis in squirrel monkeys (Saimiri sciureus) obtained as wild catches from the Amazon region were described. Histopathological evaluation of the liver, spleen, kidneys and mesenteric lymph nodes showed multiple foci of inflammation and necrosis. Typical, well-defined morphologic Toxoplasma gondii cysts and free forms of the parasite were found by immunoperoxidase staining within inflammatory infiltrates. The presence of T. gondii in the studied colony could severely influence the results of experiments performed in the animals. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Squirrel monkeys; Neotropical primates; Toxoplasmosis; Immunohistochemical analysis; Serology. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600034 |
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| Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.. |
| This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015 |
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| Bulos,L.H.S.; Gonzaga,N.F.; Souza,L.F.L.; Assao,V.S.; Leite,R.A.S.; Rebouças,M.S.; Scatamburlo,T.M.; Guimarães,J.D. Guimarães S.E.F.; Lopes,P.S.; Almeida,M.R.L.; Silva Júnior,A.. |
| ABSTRACT Brazilian pig population is made up of several naturalized breeds; among them the Piau breed is known for its rusticity and large fat stores. The naturalized breeds, in comparison with commercial ones, may have an increased resistance to diseases circulating in their territory. Thus, this study aimed to verify if there are differences between the serologic profile against Porcine circovirus 2 (PCV2) of Piau pigs and that of a commercial breed from a farm naturally infected by PCV2. The serum viral load was measured by qPCR, and levels of anti-PCV2 antibodies were measured by ELISA. The results showed that the serum viral load was similar across all animals. However, Piau piglets showed higher levels of antibodies compared to commercial piglets (P=... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serum viral load; Serology; Piau. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501145 |
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| Castro Filho,RPL; Torres,ACD; Marin,SY; Diniz,SA; Matos,JCC; Mureb,EN; Resende,M; Martins,NRS. |
| ABSTRACT Serum samples (n=687) from Gallus gallus domesticus were collected for the investigation of antibodies to avian influenza virus (AIV-A) in the family poultry of the surrounding counties of Santa Maria/RS and the metropolitan region of Belo Horizonte/MG, totaling twenty different counties. Additional samples of seventeen (n=17) free-flying ducks (C. moschata pure or hybrid with Anas platyrhynchos) were collected in Belo Horizonte. The chosen tests for the survey were performed as described by the World Organization for Animal Health (OIE), including agar gel immunodiffusion (AGID) for antibodies to AIV-A nucleoprotein (N) and haemagglutination- inhibition (HI) for antibodies to subtype H1. Out of the 704 serum tests performed by AGID, eight (8/704)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anas platyrhynchos; Avian influenza; Brazil; Cairina moschata; Family poultry; Gallus gallus domesticus; Serology. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400811 |
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| Caterino-de-Araujo,Adele; Manuel,Rolanda Carmen Rafael; Bianco,Rosana Del; Santos-Fortuna,Elizabeth; Magri,Mariana Cavalheiro; Silva,Joyce Matie Kinoshita; Bastos,Rui. |
| Human herpesvirus 8 (HHV-8), the etiological agent of Kaposi's sarcoma (KS), is endemic in parts of the sub-Saharan, and KS has increased concomitantly with the HIV/AIDS epidemic. In Mozambique (MZ), no data concerning HHV-8 infection was available, thus the main of this work was to investigate, for the first time, the presence of HHV-8 infection in Maputo, MZ. Latent and lytic HHV-8-specific antibodies were assessed in blood samples from 189 individuals from the Central Hospital of Maputo, MZ, using "in-house" immunofluorescence assays conducted in São Paulo, Brazil. The results obtained were analyzed according to socio-demographic and clinical variables using the Chi-square test and logistic regression. An HHV-8 seropositivity of 1.8% and 9.7% was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human herpesvirus 8 (HHV-8); Serology; Mozambique; Frequency; Kaposi's sarcoma. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000300008 |
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| Cavalcanti,Marta G; Gonçalves,Margareth MI; Barreto,Magali M; Silva,Aline Helen da; Madi,Kalil; Peralta,José Mauro; Igreja,Ricardo P. |
| Genital infection by Schistosoma mansoni is usually misdiagnosed in individuals who reside in, or travel to endemic areas. We describe two cases of genital tumor associated with S. mansoni infection manifested by methrorragy. Surgical specimens revealed leiomyomas in both cases associated with S. mansoni. In one of them, granulomas were found in the ovary and in the other they were found in the uterine tube. Although none presented intestinal/hepatic disease, fecal egg excretion was detected in one. Both had elevated pretreatment antibody reactivity to S. mansoni antigen, but follow-up showed different outcomes. Schistosomiasis should be considered as a diagnosis in individuals with methrorragy residing in or having traveled to endemic areas. Since... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: S. mansoni; Female genital infection; Serology; Low endemic area. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200016 |
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| Ceran,Nurgul; Turkoglu,Recai; Erdem,Ilknur; Inan,Asuman; Engin,Derya; Tireli,Hulya; Goktas,Pasa. |
| Brucellosis is a zoonotic infection and has endemic characteristics. Neurobrucellosis is an uncommon complication of this infection. The aim of this study was to present unusual clinical manifestations and to discuss the management and outcome of a series of 18 neurobrucellosis cases. Initial clinical manifestations consist of pseudotumor cerebri in one case, white matter lesions and demyelinating syndrome in three cases, intracranial granuloma in one case, transverse myelitis in two cases, sagittal sinus thrombosis in one case, spinal arachnoiditis in one case, intracranial vasculitis in one case, in addition to meningitis in all cases. Eleven patients were male and seven were female. The most prevalent symptoms were headache (83%) and fever (44%). All... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neurobrucellosis; Serology; Diagnosis; Neuroimaging. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100010 |
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| Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.. |
| In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012 |
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| Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José. |
| ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451 |
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| Cruz Romero, Anabel. |
| Los objetivos de esta investigación fueron determinar la distribución espacial e identificar los factores de riesgo asociados a la leptospirosis bovina en el estado de Veracruz, México. Se obtuvieron muestras de sangre de 3,454 bovinos de 54 municipios de las regiones Norte, Centro y Sur del estado, de febrero 2008 a diciembre de 2011. Los sueros se analizaron por medio de la técnica de aglutinación microscópica utilizando 12 serovariedades de Leptospira interrogans, se estimaron las seroprevalencias y los factores de riesgo. La seroprevalencia general fue del 3.9%; sin embargo, entre municipios varió de 0 y 44%. La mayoría de las UP con casos positivos se encontraron en la zona centro de Veracruz, y en los municipios de Cuitláhuac, Papantla, Tlacotalpan... |
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Palavras-chave: Estudio transversal; Bovinos; Serología; Epidemiología; Cross-sectional study; Bovines; Serology; Epidemiology; Agroecosistemas Tropicales; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/2336 |
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| De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da. |
| Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dengue; Diagnosis; Serology; PCR. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002 |
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| FACCIOLI-MARTINS, P. Y.; PINHEIRO, R. R.; ALVES, F. S. F.; SANTOS, V. W. S. dos; ANDRIOLI, A.; CAVALCANTE, A. C. R.; LIMA, A. M. C.; OLIVEIRA, E. L. de; ABREU, A. R. A.. |
| Apresenta o perfil zoossanitário da caprinocultura e ovinocultura de corte na região dos Inhamuns, com ênfase no município de Tauá, por meio do diagnóstico sorológico de cinco doenças infecciosas: Artrite-Encefalite Caprina (CAE), Brucelose Ovina, Língua Azul, Linfadenite Caseosa e Maedi-Visna, para respaldar ações futuras em sanidade animal junto aos produtores. |
| Tipo: Comunicado Técnico (INFOTECA-E) |
Palavras-chave: Brucelose ovina; Sorologia; Prevalência; Brasil; Região Nordeste; Sertão; Semiárido; Caprino; Ovino; Doença animal; Sanidade animal; Linfadenite caseosa; Língua Azul; Diagnostico; Goats; Sheep; Animal diseases; Caseous lymphadenitis; Disease diagnosis; Disease prevalence; Serology; Brazil; Semiarid zones. |
| Ano: 2017 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1086785 |
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| Registros recuperados: 57 | |
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